Glutamine (Gln) Colorimetric Assay Kit
Sizes: 48T, 96T
Catalogue Numbers: E-BC-K853-M-48, E-BC-K853-M-96
Citations, Manuals and MSDS Available upon request.
Target Synonym: Gln
Research Areas: Amino Acids and Proteins; Cuproptosis
Result Type: Quantitative
Detection Instrument: Microplate reader
Tested Sample Types: serum (plasma) , animal, plant tissue
Assay Time: 70 min
Detection Type: Colorimetric method
Sensitivity: 0.036 mmol/L
Detection Range: 0.036–2.0 mmol/L
Test Principle: Gln is hydrolyzed to produce glutamic acid under the action of glutaminase. Glutamic acid is further catalyzed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of Gln can be calculated by measuring the change of absorbance value at 450 nm.
Inter CV: 4.7
Intra CV: 3.2
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag