Glutathione Peroxidase 4 (GPX4) Activity Assay Kit
Sizes: 48T, 96T
Catalogue Numbers: E-BC-K883-M-48, E-BC-K883-M-96
Citations, Manuals and MSDS Available upon request.
Target Synonym: GPX4
Research Areas: Ferroptosis
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: serum, plasma, animal tissue and cell
Assay Time: 55 min
Detection Type: Colorimetric method
Sensitivity: 0.96U/L
Detection Range: 0.96-44.69U/L
Test Principle: Glutathione peroxidase 4 (GPX4) is a kind of glutathione peroxidase containing selenocysteine, which can reduce phospholipid hydroperoxide to phosphatidyl alcohol, thus protecting the cell membrane from oxidative damage.
GPX4 catalyzes the substrate, and the product consumes the reducing agent with the addition of enzyme reagents.The reducing agent has a maximum absorbance at 340 nm, and the GP4-specific enzyme activity can be calculated by measuring non-specific enzyme activity and total enzyme activity by adding GPX-4 inhibitor to the system.
Inter CV: 0.4-3.4
Intra CV: 1.2-.3.1
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag