Glycogen Colorimetric Assay Kit (Liver/Muscle Samples)
Sizes: 50 Assays, 100 Assays
Catalogue Numbers: E-BC-K073-S-50, E-BC-K073-S-100
Citations, Manuals and MSDS Available upon request.
Research Areas: Glycolysis and Lipid Metabolism
Result Type: Quantitative
Detection Instrument: Spectrophotometer
Tested Sample Types: Animal liver, muscle
Assay Time: 50 min
Detection Type: Colorimetric method
Sensitivity: 1.80 mg/g liver tiss
Detection Range: 1.80-180 mg/g liver
Test Principle: Under the presence of concentrated sulfuric acid, glycogen can be dehydrated to furfural derivatives. Furfural derivatives can form blue compound with anthracenone. The concentration of the compound can be measured by colorimetric quantification at 620 nm with glucose standard buffer of same treatment. Glycogen is quite stable in concentrated alkali solution. Heating the tissue sample in concentrated alkali solution before color development will remove other components and keep the glycogen.
Inter CV: 7.3
Intra CV: 3.7
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag