Hexokinase (HK) Activity Assay Kit
Size: 96T
Catalogue Number: E-BC-K610-M-96
Citations, Manuals and MSDS Available upon request.
Target Synonym: HK
Research Areas: Glycolysis and Lipid Metabolism
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: animal tissue, cell
Assay Time: 30 min
Detection Type: Colorimetric method
Sensitivity: 5.93 U/L
Detection Range: 5.93-40.11 U/L
Test Principle: HK converts glucose to glucose 6-phosphate, which is catalyzed by the enzyme. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of HK can be calculated by measuring the change of absorbance value at 450 nm.
Inter CV: 9.1
Intra CV: 3.3
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag