Homocysteine (Hcy) Colorimetric Assay Kit (Enzyme Circulation Method)
Size: 100 Assays
Catalogue Number: E-BC-K143-100
Citations, Manuals and MSDS Available upon request.
Target Synonym: Hcy
Research Areas: Amino Acids and Proteins
Result Type: Quantitative
Detection Instrument: Biochemistry analyzer, Spectrophotometer
Tested Sample Types: Serum
Assay Time: 25 min
Detection Type: Colorimetric method
Detection Range: 0-50 μmol/L
Test Principle: Oxidized homocysteine (HCY) is reduced to free homocysteine by triethyl phosphine (TCEP) , and the free homocysteine reacts with substrate to generate adenosine. The generated adenosine is immediately dehydrogenated into inosine and ammonia, and the ammonia is further react with NADH under the catalysis of glutamate dehydrogenase to convert NADH to NAD+. The decrease in absorbance at 340 nm caused by the decline of NADH is proportional to the concentration of homocysteine in the sample.
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag