Iron Colorimetric Assay Kit
Sizes: 48T, 96T, 500 Assays
Catalogue Numbers: E-BC-K139-M-48, E-BC-K139-M-96, E-BC-K139-M-500
Citations, Manuals and MSDS Available upon request.
Target Synonym: Fe
Research Areas: Inorganic Ions;Plant Physiology Series;Ferroptosis
Result Type: Quantitative
Detection Instrument: Microplate reader
Tested Sample Types: Serum, tissue
Assay Time: 25 min
Detection Type: Colorimetric method
Sensitivity: 0.08 mg/L
Detection Range: 0.29-10 mg/L
Test Principle: Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+) . The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.
Inter CV: 2.8
Intra CV: 1.2
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag