Lactate Dehydrogenase (LDH) Cytotoxicity Colorimetric Assay Kit
Sizes: 96T, 500 Assays
Catalogue Numbers: E-BC-K771-M-96, E-BC-K771-M-500
Citations, Manuals and MSDS Available upon request.
Target Synonym: LDH
Research Areas: Cuproptosis;Ferroptosis
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: cells
Assay Time: 30 min
Detection Type: Colorimetric method
Test Principle: Lactate dehydrogenase catalyzes the reaction of lactic acid with NAD+ to produce pyruvic acid and NADH. NADH, under the action of PMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, LDH activity can be quantified by measure the OD value at 450 nm.
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag