Mitochondrial Complex Ⅲ (Coenzyme Q-Cytochrome c Reductase) Activity Assay Kit
Sizes: 48T, 96T
Catalogue Numbers: E-BC-K151-M-48, E-BC-K151-M-96
Citations, Manuals and MSDS Available upon request.
Research Areas: Mitochondria Function;Cuproptosis
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: animal tissue
Assay Time: 10 min
Detection Type: Colorimetric method
Sensitivity: 4.45 U/L
Detection Range: 4.45–106.8 U/L
Test Principle: As an organelle, mitochondria is the "power factory" in cells and the main site of aerobic respiration of cells. Its function is to convert energy through oxidative phosphorylation to provide energy for cellular activities. The oxidation process is carried out by four respiratory chain membrane protein complexes (complexes I, II, III and IV) on the inner mitochondrial membrane. mitochondrial complex III, also known as cytochrome c reductase complex, its main function is to oxidize the reduced coenzyme Q10 formed by mitochondrial complexes I and II to oxidative coenzyme Q10. In this process, the OD value increased at 550 nm. Therefore, the activity of mitochondrial complex III can be quantified by measure the change OD value at 550 nm.
Inter CV: 10
Intra CV: 5
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag