Mitochondrial Complex Ⅴ (F0F1-ATPase/ATP Synthase) Activity Assay Kit
Sizes: 48T, 96T
Catalogue Numbers: E-BC-K153-M-48, E-BC-K153-M-96
Citations, Manuals and MSDS Available upon request.
Target Synonym: ATP Synthase
Research Areas: Mitochondria Function;Cuproptosis
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: animal tissue, cell
Assay Time: 15 min
Detection Type: Colorimetric method
Sensitivity: 12.13 U/L
Detection Range: 12.13-74.21 U/L
Test Principle: Mitochondrial complex V is also known as F0F1-ATP synthase. ATP is hydrolyzed by F0F1-ATP synthase to produce ADP, and ADP converts NADH into oxidized coenzyme I (NAD) after enzyme conversion reaction. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 340 nm.
Inter CV: 6.5
Intra CV: 5.6
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag