Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit
Sizes: 48T, 96T
Catalogue Numbers: E-BC-K149-M-48, E-BC-K149-M-96
Citations, Manuals and MSDS Available upon request.
Research Areas: Mitochondria Function;Cuproptosis
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: animal tissue
Assay Time: 15 min
Detection Type: Colorimetric method
Sensitivity: 15.58 U/L
Detection Range: 15.58-224.2 U/L
Test Principle: Mitochondrial complex I catalyzes the reaction of NADH with ubiquinone substrate to generate NAD+ and reduced ubiquinone. The specific inhibitor rotenone can inhibit the enzyme, and the total enzyme activity minus the enzyme activity after inhibitor inhibition can reflect its specific enzyme activity.
Inter CV: 8.4
Intra CV: 0.8
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag