Oxaloacetate Colorimetric Assay Kit
Sizes: 48T, 96T
Catalogue Numbers: E-BC-K901-M-48, E-BC-K901-M-96
Citations, Manuals and MSDS Available upon request.
Research Areas: Tricarboxylic Acid（TCA）Cycle
Result Type: Quantitative
Detection Instrument: Microplate reader
Tested Sample Types: animal tissue
Assay Time: 15 min
Detection Type: Colorimetric method
Sensitivity: 0.017 mmol/L
Detection Range: 0.017-1 mmol/L
Test Principle: Oxaloacetic acid (OAA) , one of the intermediates in the tricarboxylic acid cycle is an important substance in carbon and nitrogen metabolism. Oxaloacetate reacts with substrate acetyl-coA under the action of enzyme. The substance produced can react with DTNB, which has the maximum absorption peak at 412 nm. The content of oxaloacetate can be determined by measuring the absorbance value.
Inter CV: 2.3
Intra CV: 2.1
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag