Pepsin Activity Assay Kit
Size: 96T
Catalogue Number: E-BC-K844-M-96
Citations, Manuals and MSDS Available upon request.
Research Areas: Amino Acids and Proteins
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: gastric, intestinal tissue
Assay Time: 20 min
Detection Type: Colorimetric method
Sensitivity: 0.63 U/mL
Detection Range: 0.63-94.98 U/mL
Test Principle: Pepsin can catalyze substrate hydrolysis, and the hydrolysate reacts with the chromogenic agent under alkaline conditions to form a blue substance. The blue substance has a maximum absorption peak at 660 nm, and the color depth is proportional to the activity of pepsin. The enzyme activity can be calculated by OD value
Inter CV: 4.8-5.6
Intra CV: 1.3-2.2
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag