Phosphofructokinase（PFK）Activity Assay Kit
Sizes: 48T, 96T
Catalogue Numbers: E-BC-K612-M-48, E-BC-K612-M-96
Citations, Manuals and MSDS Available upon request.
Target Synonym: PFK
Research Areas: Glycolysis and Lipid Metabolism
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: serum, plasma, tissue and cell sample
Assay Time: 15 min
Detection Type: Colorimetric method
Sensitivity: 0.27 U/L
Detection Range: 0.27-32.29 U/L
Test Principle: Phosphofructokinase (also known as 6-phosphofructokinase; PFK) is a class of kinases that act on fructose-6-phosphate. Phosphofructokinase catalyzes fructose-6-phosphate and ATP to produce fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+. The activity of PFK can be reflected by the determination of NADH decline rate at 340 nm.
Inter CV: 8
Intra CV: 3
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag