Pyruvate Decarboxylase (PDC) Activity Assay Kit
Sizes: 48T, 96T
Catalogue Numbers: E-BC-K500-M-48, E-BC-K500-M-96
Citations, Manuals and MSDS Available upon request.
Target Synonym: PDC
Research Areas: Glycolysis and Lipid Metabolism
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: serum, plasma, tissue and cell samples
Assay Time: 15 min
Detection Type: Colorimetric method
Sensitivity: 0.67 U/L
Detection Range: 0.67-27.73 U/L
Test Principle: Pyruvate decarboxylase (PDC) mainly exists in yeast and is one of the key enzymes in ethanol fermentation. PDC catalyzes pyruvate decarboxylate to acetaldehyde, which reacts under the action of ethanol dehydrogenase (ADH) , and catalyzes NADH to convert into NAD+. NADH has a characteristic absorption peak at 340 nm. The PDC activity can be calculated by measuring the OD value at 340 nm.
Inter CV: 3.1
Intra CV: 2.8
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag