Pyruvate Dehydrogenase (PDH) Activity Assay Kit
Size: 96T
Catalogue Number: E-BC-K650-M-96
Citations, Manuals and MSDS Available upon request.
Target Synonym: PDH
Research Areas: Tricarboxylic Acid（TCA）Cycle
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: serum (plasma) , urine, animal tissue, cell
Assay Time: 15 min
Detection Type: Colorimetric method
Sensitivity: 0.28 U /L
Detection Range: 0.28-76.23 U/L
Test Principle: Pyruvate dehydrogenase (PDH) catalyzed the oxidative decarylation of pyruvate into acetyl CoA, which plays an important role in the metabolism of mitochondrial respiratory chain and is associated with the aging of cancer genes. Pyruvate can be decarboxylated by PDH under the action of CoA. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of PDH can be calculated by measuring the change of absorbance value at 450 nm.
Inter CV: 10
Intra CV: 2
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag