Succinate Dehydrogenase (SDH) Activity Assay Kit
Sizes: 48T, 96T
Catalogue Numbers: E-BC-K649-M-48, E-BC-K631-M-96
Citations, Manuals and MSDS Available upon request.
Target Synonym: SDH
Research Areas: Mitochondria Function;Tricarboxylic Acid（TCA）Cycle
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: animal tissue, cell
Assay Time: 10 min
Detection Type: Colorimetric method
Sensitivity: 0.83 U/L
Detection Range: 0.83-65.42 U/L
Test Principle: SDH catalyzes the dehydrogenation of succinate to fumarate, with electron transport materials transferring electrons to 2, 6-dichlorophenol indiophenol (DCPIP) . Then, the reduced DCPIP is reduced to the oxidized DCPIP, which has a characteristic absorption peak at 600 nm. Therefore, the activity of SDH can be quantified by measure the change OD value at 600 nm.
Inter CV: 4
Intra CV: 2
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag