Sucrase Activity Assay Kit
Size: 96T
Catalogue Number: E-BC-K751-M-96
Citations, Manuals and MSDS Available upon request.
Research Areas: Inorganic Ions;Plant Physiology Series;Inorganic Ions
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: animal tissu
Assay Time: 70 min
Detection Type: Colorimetric method
Sensitivity: 20 U/mL
Detection Range: 20-2000 U/mL
Test Principle: Sucrase catalyzes its substrate (sucrose) to produce glucose, which produces hydrogen peroxide under the action of glucose oxidase. Hydrogen peroxide reacts with chromogenic agent to produce red substance, which has a strong absorption peak at 505 nm. In a certain concentration range, It's absorbance is proportional to glucose concentration. Therefore, the activity of sucrase can be calculated by measuring the OD value at 505 nm.
Inter CV: 6.5
Intra CV: 5.4
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag