Sucrose Colorimetric Assay Kit
Sizes: 50 Assays, 100 Assays
Catalogue Numbers: E-BC-K161-S-50, E-BC-K161-S-100
Citations, Manuals and MSDS Available upon request.
Research Areas: Glycolysis and Lipid Metabolism
Result Type: Quantitative
Detection Instrument: Spectrophotometer
Tested Sample Types: Plant tissue
Assay Time: 40 min
Detection Type: Colorimetric method
Sensitivity: 0.32 μmol/mL
Detection Range: 0.32-70 μmol/mL
Test Principle: Sucrose in plant tissue is hydrolyzed to glucose and fructose in boiling water bath under acidic conditions. 5-hydroxymethyl furfural was synthesized from fructose under acid condition and measure the ultraviolet absorption of 5-hydroxymethyl furfural. Glucose must be dissimilated into ketose structure and reduced to obtain 5-hydroxymethylfurfural, but the rate of isomerization of glucose to ketose is very slow. Therefore, the ultraviolet absorption of glucose is much smaller than fructose.
Inter CV: 8.5
Intra CV: 3.4
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag