Total Bile Acid (TBA) Colorimetric Assay Kit
Sizes: 48T, 96T, 500 Assays
Catalogue Numbers: E-BC-K181-M-48, E-BC-K181-M-96, E-BC-K181-M-500
Citations, Manuals and MSDS Available upon request.
Target Synonym: TBA
Research Areas: Liver and Renal Function
Result Type: Quantitative
Detection Instrument: Microplate reader
Tested Sample Types: serum, animal tissue
Assay Time: 25 min
Detection Type: Colorimetric method
Sensitivity: 2.05 μmol/L
Detection Range: 2.05-120 μmol/L
Test Principle: With S-NAD+ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.
Inter CV: 3.7
Intra CV: 3.4
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag