Urea (BUN) Colorimetric Assay Kit (Diacetyl Oxime Method)
Sizes: 50 Assays, 100 Assays
Catalogue Numbers: E-BC-K329-S-50, E-BC-K329-S-100
Citations, Manuals and MSDS Available upon request.
Target Synonym: BUN
Research Areas: Liver and Renal Function
Result Type: Quantitative
Detection Instrument: Spectrophotometer
Tested Sample Types: Serum, plasma, saliva, urine
Assay Time: 60 min
Detection Type: Colorimetric method
Sensitivity: 0.12 mmol/L
Detection Range: 0.12-15 mmol/L
Test Principle: In strong acidic and heating condition, urea can react with diacetyl to form red diazine compound. The depth of color is proportional to the content of urea. Because the instability of the diacetyl, the diacetyl oxime usually react with the strong acid firstly in the reaction system to generate diacetyl, then react with urea to generate the red diazine compound. The reaction equation is as follows:
Inter CV: 9.9
Intra CV: 4.9
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag