pan-Tri-Methyl lysine Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A20145-20, A20145-100
Citations, Manuals and MSDS Available upon request.
Background: Methylation of lysine residues is a common regulatory post-translational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs). The post-translational ε-amino lysine methylated proteins is an important reversible modification which plays a vital role in the regulation of many cellular processes including chromatin dynamics and gene transcription. Methylation of lysine residues is modulated by specific counteractive enzymes including lysine methylases (KMTs) and demethylases (KDMs). Lysine trimethylation occurs in both histones and non-histone substratres. It has become promising targets for discovery of anti-cancer drugs.
Applications: Methylated Antibodies
Applications: WB, IP, ELISA, ChIP
Cross-reactivity: Human, Mouse, Rat, Other (Wide Range Predicted)
Observed Molecular Weight: 15-74kDa
Immunogen: Synthetic peptide
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP, 3μg antibody for 5μg-10μg of Chromatin
NCBI Alias: pan Kme3
Research Use Only