Visualizing lipofection
Catalogue Number: K020-0.1 (1 x 100μl)
Size: 1 x 100μl (K020-0.1)
HIGHLIGHTS:
- Ideal optical properties for transmitted light and fluorescence microscopy
- Visualization of transfection process in living cells
- Simple transfection protocol (no optimization necessary)
- DNA and siRNA transfection
- Cells can be fixed and dyed
- Compatible with oil immersion
Product Specification
| Application | Visualization of lipofection by fluorescence and high resolution microscopy of living mammalian cells (live cell imaging) |
|---|---|
| Content | METAFECTENE® µ FluoR 1 x PBS µ-Slides VI (6 channels each) |
| Assays | 90 per kit |
| Shipping | At room temperature |
| Storage | 4°C |
| Shelf life | 1 year (after date of delivery) |
| Product Group | Microfection |
μ-Transfection Kit VI FluoR is the ideal solution where visualization of the carrier system's path through the cell is necessary, for example to gain information about the transfection process.
In addition to all the benefits of the μ-Transfection Kit VI FluoR, this kit offers the option of tracking lipoplexes in living cells. For this purpose the transfection reagent METAFECTENE® μ was labeled with the fluorescent dye rhodamine as METAFECTENE® μ FluoR, while retaining its other properties. This enables the transfection process itself to be tracked microscopically.
High-resolution microscopic view of HepG2 cells transfected with the μ-Transfection Kit VI FluoR. The images clearly show the fluorescent red-marked lipoplexes taken up by endocytosis and the incipient expression of the fluorescent green GFP. (Images of the individual fluorescence channels and layered images).