Human COL5a2 (Collagen Type V Alpha 2) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK3965MS-48, ELK3965MS-96, ELK3965MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: CENP-F; CENF; Hcp-1; Centromere protein F (350/400kD, Mitosin); AH antigen; Kinetochore protein CENPF; Mitosin
Assay Type: Sandwich
Sensitivity: 0.054 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Areas: Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CENPF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CENPF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CENPF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CENPF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P49454
Research Use Only