Human DEFa6 (Defensin Alpha 6, Paneth Cell Specific) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK3747MS-48, ELK3747MS-96, ELK3747MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: CD227; EMA; H23AG; KL-6; PEM; PEMT; PUM; CA15-3; Cancer antigen 15-3; Carcinoma-associated mucin; Episialin; Peanut-reactive urinary mucin; Polymorphic epithelial mucin
Assay Type: Sandwich
Sensitivity: 0.064 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Tumor immunity; Infection immunity; Pulmonology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MUC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MUC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MUC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MUC1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q02496
Research Use Only