Human ESM1 (Endothelial Cell Specific Molecule 1) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK3625MS-48, ELK3625MS-96, ELK3625MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: IHBC; INH-Bc
Assay Type: Sandwich
Sensitivity: 26 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Areas: Endocrinology; Reproductive science; Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat INHbC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat INHbC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat INHbC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat INHbC in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9WUK5
Research Use Only