Human F8 (Coagulation Factor VIII) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10386MS-48, ELK10386MS-96, ELK10386MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: COL7-A1; COL7A1; EBD1; EBDCT; EBR1; Long-chain collagen; Epidermolysis Bullosa, Dystrophic, Dominant And Recessive; Collagen Alpha-1 (VII)chain
Assay Type: Sandwich
Sensitivity: 5.7 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Areas: Metabolic pathway; Developmental science; Nutrition metabolism; Bone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL7 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only