Human Grb10 (Growth Factor Receptor Bound Protein 10) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK3635MS-48, ELK3635MS-96, ELK3635MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: PROKR1; PKR-1; GPR73; GPR-73a; ZAQ; PK-R1; G Protein-Coupled Receptor 73; G-protein coupled receptor ZAQ
Assay Type: Sandwich
Sensitivity: 0.221 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Areas: Metabolic pathway; Developmental science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PKR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PKR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PKR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PKR1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q8TCW9
Research Use Only