Human IREM1 (Immune Receptor Expressed On Myeloid Cells 1) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK3899MS-48, ELK3899MS-96, ELK3899MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: CD162; SELPLG; CLA; Selectin P Ligand
Assay Type: Sandwich
Sensitivity: 0.07 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Areas: CD and Adhesion molecule; Tumor immunity; Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PSGL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PSGL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PSGL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PSGL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q62170
Research Use Only