Human PAR4 (Protease Activated Receptor 4) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK3745MS-48, ELK3745MS-96, ELK3745MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: S100-A1; S100A1; S100-Alpha; S-100 Protein; S-100 protein subunit alpha; S-100 protein alpha chain
Assay Type: Sandwich
Sensitivity: 0.62 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human S100. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S100. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human S100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human S100 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: B2R5H0
Research Use Only