Human S100A12 (S100 Calcium Binding Protein A12) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK1073MS-48, ELK1073MS-96, ELK1073MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: TP; PMFA; VEGP; Tlc; Tear Prealbumin; Tear lipocalin; Protein Migrating Faster Than Albumin; Von Ebner Gland Protein
Assay Type: Sandwich
Sensitivity: 0.25 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: serum, plasma, saliva, tears and other biological fluids
Assay Length: 3.5h
Research Areas: Metabolic pathway; Infection immunity; Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LCN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LCN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LCN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LCN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only