Human TNFa (Tumor Necrosis Factor Alpha) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK1190MS-48, ELK1190MS-96, ELK1190MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: INH-BB; Activin AB Beta Polypeptide
Assay Type: Sandwich
Sensitivity: 4.72 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Areas: Tumor immunity; Endocrinology; Reproductive science; Developmental science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse INHbB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse INHbB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse INHbB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse INHbB in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q04999
Research Use Only