Mouse CASP3 (Caspase 3) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK1529MS-48, ELK1529MS-96, ELK1529MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Mouse
Alternative Names: SKP1A; EMC19; OCP-II; OCP2; TCEB1L; p19A; Cyclin-A/CDK2-associated p19; RNA polymerase II elongation factor-like protein; Transcription elongation factor B polypeptide 1-like
Assay Type: Sandwich
Sensitivity: 0.056 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Areas: Enzyme and Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SKP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SKP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SKP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SKP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9WTX5
Research Use Only