Mouse CD34 (Cluster Of Differentiation 34) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK2708MS-48, ELK2708MS-96, ELK2708MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Mouse
Alternative Names: CD292; ACVRLK3; ALK3; SKR5; BMPR1-A; Activin A Receptor, Type Ii-Like Kinase 3; Activin Receptor-Like Kinase 3; Serine/Threonine-Protein Kinase Receptor R5
Assay Type: Sandwich
Sensitivity: 0.25 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Areas: Signal transduction; CD and Adhesion molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BMPR1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BMPR1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BMPR1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BMPR1A in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q78EA7
Research Use Only