Mouse DEFa5 (Defensin Alpha 5, Paneth Cell Specific) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK2462MS-48, ELK2462MS-96, ELK2462MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Mouse
Alternative Names: Apo-H; B2G1; BG; B2-GP1; B2GP1; Previously; Beta 2 Glycoprotein 1; APC inhibitor; Activated protein C-binding protein; Anticardiolipin cofactor; Beta (2)GPI
Assay Type: Sandwich
Sensitivity: 1.8 µg/mL
Standard: 500 µg/mL
Detection Range: 7.82-500 µg/mL
Sample Type: Serum, plasma, saliva and other biological fluids.
Assay Length: 3.5h
Research Areas: Metabolic pathway; Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse APOH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APOH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse APOH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APOH in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q01339
Research Use Only