Mouse LBP (Lipopolysaccharide Binding Protein) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10482MS-48, ELK10482MS-96, ELK10482MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Mouse
Alternative Names: Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc
Assay Type: Sandwich
Sensitivity: 2.23 ng/mL
Standard: 500 ng/mL
Detection Range: 7.81-500 ng/mL
Sample Type: plasma
Assay Length: 3.5h
Research Areas: Diagnosis of diabetes mellitus
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HbA1c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HbA1c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only