Rat EPOR (Erythropoietin Receptor) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK2481MS-48, ELK2481MS-96, ELK2481MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Rat
Alternative Names: ApoA-IV; ApoAIV; Apo-A4
Assay Type: Sandwich
Sensitivity: 0.92 µg/mL
Standard: 200 µg/mL
Detection Range: 3.13-200 µg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Areas: Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Gastroenterology; Nutrition metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat APOA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APOA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat APOA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APOA4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P02651
Research Use Only