ABflo® 594 Rabbit anti-Human ICAM-1/CD54 Monoclonal Antibody
Sizes: 100T, 2 x 100T, 5 x 100T
Catalogue Numbers: A24587-100, A24587-200, A24587-500
Citations, Manuals and MSDS Available upon request.
Background: Intercellular cell adhesion molecule-1 (CD54 or ICAM-1) is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules. CD54 is expressed at low levels in diverse cell types, and is induced by cytokines (TNF-α, interleukin-1) and bacterial lipopolysaccharide . Apical localization of CD54 on endothelial cells (or basolateral localization on epithelial cells) is a prerequisite for leukocyte trafficking through the endothelial (or epithelial) barrier . Apical expression of CD54 on epithelial cells mediates pathogen invasion as well as host defense, a pattern also observed in tumors . CD54 also functions as a co-stimulator on antigen presenting cells, binding to its receptor LFA-1 (leukocyte function-associated antigen-1) on the surface of T cells during antigen presentation . Cross-linking of CD54 or binding to its ligand triggers activation of Src family kinases and the Rho/ROCK pathway . Phosphorylation on Tyr485 of CD54 is required for its association with SHP-2. SHP-2 seems essential for CD54-induced Src activation.
Category: Monoclonal Antibodies
Application: FC
Cross Reactivity: Human
Protein Weight: 57kDa Immunogen: Recombinant protein (or fragment).
Species: Human
GeneID: 3383
SWISS: P05362
Alternate Names: ICAM1; BB2; CD54; P3.58; intercellular adhesion molecule 1
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at 2-8℃. Avoid freeze.|Buffer: PBS containing 0.2% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilution: FC,5 μl per 10^6 cells in 100 μl volume
Research Areas: Cell Biology and Developmental Biology, Cell Adhesion, Cell Adhesion Molecules, Immunology and Inflammation, CD markers, Neuroscience, Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease, Neurodegenerative Diseases Markers, Stem Cells, Hematopoietic Progenitors, Cardiovascular
NCBI Alias: ICAM1
Modifications: Unmodified
Conjugate: ABflo® 594. Ex:588nm. Em:604nm.
Clone Number Info: ARC54998
Research Use Only