APAF1 Rabbit Monoclonal Antibody
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Sizes: 50µl, 100µl
Catalogue Numbers: BS40093-50, BS40093-100
Swiss-Prot: O14727
Host: Rabbit
APAF1 Rabbit Monoclonal Antibody -BS40093
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Reactivity: Human, Mouse
Applications: WB, IHC, ICC/IF
All Applications: WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200
Background: APAF Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Induced by E2F and p53 in apoptotic neurons. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. 6 isoforms of the human protein are produced by alternative splicing.
Purification and Purity: Affinity-chromatography
Specificity: APAF1 Antibody detects endogenous levels of total APAF1
Bioworld Molecular Weight: ~ 130 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of APAF1 Rabbit monoclonal antibody at 1:500 dilution
Lane 1: MCF-7 whole cell lysate (30ug)
Lane 2: HepG2 whole cell lysate (30ug), Immunohistochemical analysis of paraffin-embedded mouse kidney, using APAF1 Antibody.
Alternative Name: APAF; APAF-1; APAF1; apoptotic peptidase activating factor 1; apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; CED4; DKFZp781B1145; KIAA0413
Immunogen: A synthesized peptide derived from human APAF1
Modification: Unmodified
Conjugate: Unconjugated