BUBR1 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB11940-50, MB11940-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: O60566
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IP,
All Applications: WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
Background: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 120 kDa; Observed MW: 120 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of BubR1 in Hela, A549, MCF-7, U251 lysates using BUBR1 antibody., Immunocytochemistry analysis of BubR1 in hela using BubR1 antibody, and DAPI
Alternative Name: BUB1B; BUBR1; MAD3L; SSK1; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1
Immunogen: A synthetic peptide of human BubR1
Conjugate: Unconjugated
Modification: Unmodified