Calreticulin Monoclonal Antibody
Catalogue Numbers: MB66950-50, MB66950-100
Sizes: 50µl, 100µl
Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Swiss-Prot: P27797
Host: Mouse
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB (1/500 - 1/1000)
Background: Calcium is a universal signaling molecule involved in many cellular functions such as cell motility, metabolism, protein modification, protein folding, and apoptosis. Calcium is stored in the endoplasmic reticulum (ER), where it is buffered by calcium binding chaperones such as calnexin and calreticulin, and is released via the IP3 Receptor channel . Calreticulin also functions as an ER chaperone that ensures proper folding and quality control of newly synthesized glycoproteins. As such, calreticulin presumably does not alter protein folding but regulates proper timing for efficient folding and subunit assembly. Furthermore, calreticulin retains proteins in non-native conformation within the ER and targets them for degradation .
Purification and Purity: This antibody is purified through a protein G column.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of Calreticulin protein.
Extra Notes: Western blot analysis of Calreticulin expression in HepG2 (A), Jurkat (B), HeLa (C), MCF7 (D), SHSY5Y (E), K562 (F), NIH3T3 (G), C6 (H), CHO (I) whole cell lysates.
Bioworld Molecular Weight: ~ 60 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: CRTC; Calreticulin; CRP55; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP; grp60
Immunogen: Recombinant fusion protein of human Calreticulin. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified