CaMKIα (S173) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS1728-50, BS1728-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q14012
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
Background: The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated α, β, γ and δ, which may or may not be coexpressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal, leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory threonine residue at position 177.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunohistochemistry (IHC) analyzes of CaMKIα (S173) pAb in paraffin-embedded human colorectal cancer carcinoma tissue at 1:100.
Bioworld Molecular Weight: ~ 41 kDa
Specificity: CaMKIα (S173) polyclonal antibody detects endogenous levels of CaMKIα protein.
Alternative Name: CaM K1; CaM KI; CaM kinase 1 alpha; CAM kinase 1; CaM kinase I alpha; CAM kinase I; CaMK 1; CAMK I; CaMK1 alpha; CaMK1; CAMK1 PEN; CaMKI alpha; MGC120317; MGC120318;
Immunogen: Synthetic peptide, corresponding to amino acids 140-192 of Human CaMKIα.
Conjugate: Unconjugated
Modification: Unmodified