Caspase 3 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB10383-50, MB10383-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P42574
Host: Rabbit
Reactivity: Human
Applications: WB, IHC-F, IHC-P, ICC/IF, IP,
All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Background: Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 32 kDa; Observed MW: 32 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunocytochemistry analysis of Caspase 3 in 293 using Caspase 3 antibody, and DAPI., Western blot analysis of Caspase3 in K562, Hela lysates using Caspase3 antibody., Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Caspase3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Alternative Name: CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
Immunogen: A synthetic peptide of human Caspase-3
Conjugate: Unconjugated
Modification: Unmodified