CBR1 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB66547-50, MB66547-100
Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Swiss-Prot: P16152
Host: Mouse
Reactivity: Human
Applications: WB, IF/ICC
All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)
Background: NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol.Can convert prostaglandin E to prostaglandin F2-alpha (By similarity).Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.In addition, participates in the glucocorticoid metabolism by catalyzing the NADPH-dependent cortisol/corticosterone into 20beta-dihydrocortisol (20b-DHF) or 20beta-corticosterone (20b-DHB), which are weak agonists of NR3C1 and NR3C2 in adipose tissue.
Purification and Purity: The antibody was purified by immunogen affinity chromatography.
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Recognizes endogenous levels of CBR1 protein.
Bioworld Molecular Weight: ~ 28 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of CBR1 expression in Hela (A), A431 (B), MDAMB468 (C) whole cell lysates., Immunofluorescent analysis of CBR1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Alternative Name: CBR; CRN; Carbonyl reductase [NADPH] 1; 15-hydroxyprostaglandin dehydrogenase [NADP(+)]; NADPH-dependent carbonyl reductase 1; Prostaglandin 9-ketoreductase; Prostaglandin-E(2) 9-reductase
Immunogen: Purified recombinant human CBR1 protein fragments expressed in E.coli
Conjugate: Unconjugated
Modification: Unmodified