Collagen IV Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: A25131-20, A25131-100
Citations, Manuals and SDS Available upon request.
Background: Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.
Synonyms: ATS2; ATS3; BFH2; COL4A3/COL4A5/COL4A2/COL4A1
GeneID: 1285/1287/1284/1282
Swiss-Prot: Q01955
Reactivity: Human, Rat
Tested Applications: ELISA, WB, IF/ICC
Calculated Molecular Weight: 162kDa
Observed Molecular Weight: 160-200kDa
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1555-1669 of human COL4A3 (NP_000082.2).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Research Areas: Cancer, Invasion and Metastasis, Signal Transduction, Cell Biology Developmental Biology, Apoptosis, Cytoskeleton, Extracellular Matrix, Immunology Inflammation, Cardiovascular, Angiogenesis
Recommended Dilutions: WB: 1:500 - 1:1000 | IF/ICC: 1:50 - 1:200
Modifications: Unmodified
Conjugate: Unconjugated
Cellular Location: Secreted, basement Membrane, Extra Cellular Matrix, Extra Cellular Space
Positive Samples: HeLa, U266
Keywords: 1285, COL4A3, collagen type IV alpha 3 chain, collagen alpha-3(IV) chain, collagen IV, alpha-3 polypeptide, collagen, type IV, alpha 3 (Goodpasture antigen), tumstatin, Q01955, Collagen Type IV Alpha 3 Chain, Collagen, Type IV, Alpha 3 (Goodpasture Antigen), Tumstatin, Collagen IV, Alpha-3 Polypeptide, Collagen Alpha-3(IV) Chain, Goodpasture Antigen
Symbol: COL4A3/COL4A5/COL4A2/COL4A1
Category: Monoclonal Antibodies
Research Use Only