[KD Validated] PKA C-alpha (PRKACA) Mouse mAb - A18603

[KD Validated] PKA C-alpha (PRKACA) Mouse mAb

Sizes: 20μL, 100μL

Catalogue Numbers: A18603-20, A18603-100

Citations, Manuals and MSDS Available upon request.

Background: This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.

Category: Monoclonal Antibodies

Applications: WB, IF/ICC, ELISA

Cross-reactivity: Human, Mouse, Rat

Protein Weight: 41kDa

Observed Molecular Weight: 40kDa

Immunogen: Recombinant protein (or fragment) .

Species: Human

GeneID: 5566

SWISS: P17612

Alternate Names: CAFD1; PKACA; PPNAD4; A)

Source: Mouse

Isotype: IgG2B, Kappa

Purity: Affinity purification

Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis),  pH 7.3.

Recommended Dilutions: WB, 1:10000 - 1:40000 IF/ICC, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Research Areas: Protein phosphorylation, Cancer, Signal Transduction, G protein signaling, G-Protein-Coupled Receptors Signaling to MAPK Erk, Kinase, Serine threonine kinases, MAPK-Erk Signaling Pathway, Cell Biology Developmental Biology, Apoptosis, Mitochondrial Control of Apoptosis, Inhibition of Apoptosis, Cell Cycle, Centrosome, Cytoskeleton, Microtubules, Actins, Endocrine Metabolism, Lipid Metabolism, Carbohydrate metabolism, AMPK Signaling Pathway, Insulin Receptor Signaling Pathway, Immunology Inflammation, NF-kB Signaling Pathway, Neuroscience, Neurodegenerative Diseases, Dopamine Signaling in Parkinson's Disease.

NCBI Alias: PRKACA

Research Use Only