[KO Validated] AKT1 Rabbit mAb - A22770

[KO Validated] AKT1 Rabbit mAb

Sizes: 20µl, 100µl

Catalogue Numbers: A22770-20, A22770-100

Citations, Manuals and SDS Available upon request.

Background: This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene.

Synonyms: AKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA; T1

GeneID: 207

Swiss-Prot: P31749

Reactivity: Human, Mouse, Rat

Tested Applications: ELISA, WB, IHC-P, IF/ICC

Calculated Molecular Weight: 56kDa

Observed Molecular Weight: 60kDa

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 380-480 of human AKT1 (NP_005154.2).

Immunogen Species: Human

Host: Rabbit

Isotype: IgG

Purity: Affinity purification

Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.

Research Areas: Epigenetics Nuclear Signaling, Translation Control, Regulation of eIF4 and p70 S6 Kinase, Protein phosphorylation, Cancer, Signal Transduction, G protein signaling, Kinase, Serine threonine kinases, PI3K-Akt Signaling Pathway, mTOR Signaling Pathway, ErbB-HER Signaling Pathway, Cell Biology Developmental Biology, Apoptosis, Mitochondrial Control of Apoptosis, Inhibition of Apoptosis, Cell Cycle, Cell Cycle Control-G1 S Checkpoint, Cell Adhesion, Microtubules, TGF-b-Smad Signaling Pathway, ESC Pluripotency and Differentiation, Endocrine Metabolism, AMPK Signaling Pathway, Insulin Receptor Signaling Pathway, Warburg Effect, Immunology Inflammation, B Cell Receptor Signaling Pathway, T Cell Receptor Signaling Pathway, Jak-Stat-IL-6 Receptor Signaling Pathway, NF-kB Signaling Pathway, Neuroscience, Neurodegenerative Diseases, Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease, Cardiovascular, Angiogenesis

Recommended Dilutions: WB: 1:500 - 1:1000 | IHC-P: 1:50 - 1:200 | IF/ICC: 1:50 - 1:200

Modifications: Unmodified

Conjugate: Unconjugated

Cellular Location: Cell Membrane, Cytoplasm, Nucleus

Positive Samples: MCF7, Hela, Mouse testis, Mouse brain, Mouse thymus, Rat testis

Keywords: 207, sc-7126, sc-1619, sc-1618, sc-55523, sc-8312, AKT, AKT (pan), AKT pan, AKT1, AKT1/2/3, pan AKT, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha, total AKT, AKT1-phospho-S473, pakt, p-AKT, Phospho akt, AKT2, AKT3, CWS6, PKB-ALPHA, RAC-ALPHA, AKT serine/threonine kinase 1, RAC-alpha serine/threonine-protein kinase, AKT1m, PKB alpha, RAC-PK-alpha, protein kinase B alpha, proto-oncogene c-Akt, rac protein kinase alpha, serine-threonine protein kinase, v-akt murine thymoma viral oncogene homolog 1, v-akt murine thymoma viral oncogene-like protein 1, P31749, AKT Serine/Threonine Kinase 1, V-Akt Murine Thymoma Viral Oncogene Homolog 1, Protein Kinase B Alpha, Proto-Oncogene C-Akt, Protein Kinase B, RAC-PK-Alpha, PKB Alpha, V-Akt Murine Thymoma Viral Oncogene-Like Protein 1, RAC-Alpha Serine/Threonine-Protein Kinase, Serine-Threonine Protein Kinase, Rac Protein Kinase Alpha, EC 2.7.11.1, RAC-alpha serine/threonine-protein kinaseAKT1mPKB alphaRAC-PK-alphaprotein kinase B alphaproto-oncogene c-Aktrac protein kinase alphaserine-threonine protein kinasev-akt murine thymoma viral oncogene homolog 1v-akt murine thymoma viral oncogene-like protein 1, T1

Symbol: AKT1

Category: Monoclonal Antibodies

Research Use Only