p53 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB11357-50, MB11357-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P04637
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF
All Applications: WB: 1/500-1/1000 IF: 1/50-1/200
Background: Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 44 kDa; Observed MW: 53 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of p53 in Jurkat lysates using p53 antibody., Immunocytochemistry analysis of p53 in MCF-7 using p53 antibody, and DAPI
Alternative Name: TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
Immunogen: Recombinant protein of human p53
Conjugate: Unconjugated
Modification: Unmodified