Pan-Akt Rabbit mAb
Sizes: 20µl, 100µl
Catalogue Numbers: A22412-20, A22412-100
Citations, Manuals and SDS Available upon request.
Background: Human AKT serine-threonine protein kinase family includes three members AKT1, AKT2, AKT3, which are also often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3, 4, 5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4, 5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene.
GeneID: 207/ 208/ 10000
Swiss Prot: P31749/P31751/Q9Y243
Tested Applications: ELISA, WB, IHC-P, IP
Reactivity: Human, Mouse, Rat
Observed Molecular Weight: 60kDa
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-123 of human Pan-AktAKT1 (NP_005154.2).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles.|Buffer: PBS with 0.01% thimerosal, 0.05% BSA, 50% glycerol, pH7.3.
Recommended Dilutions: WB: 1:500 - 1:1000 | IHC-P: 1:50 - 1:200
Positive Samples: HeLa, MCF7, A-549, Mouse brain, Mouse testis, Rat brain, Rat testis
Cellular Location: Ciliary Basal Body, Cytoplasm, Cytosol, Microtubule Cytoskeleton, Mitochondrion, Nucleoplasm, Nucleus, Plasma Membrane, Spindle
Symbol: AKT
Category: Monoclonal Antibodies
Research Use Only