PCNA Monoclonal Antibody
Sizes: 50μL, 100μL, 200μL
Catalogue Numbers: E-AB-48013-50, E-AB-48013-100, E-AB-48013-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: PCNA
Target Synonym: ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; OTTHUMP00000030189; OTTHUMP00000030190; Pcna/cyclin; PCNAR; Polymerase delta accessory protein; Proliferating cell nuclear antigen; wu:fa28e03; wu:fb36g03
Research Areas: Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Tags and Cell Markers
Conjugation: Unconjugated
Host: Mouse
Species Reactivity: Zebrafish
Application: WB, IF, IHC
Isotype: IgG1
Clonality: Monoclonal
Clone No.: 8B12
UNIProt ID: P12004
Background: Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics. PCNA gene is often stably and constitutively expressed in most tissues and cells, it is commonly used as a loading control for western blot and other test.
Concentration: 2mg/mL
Immunogen: Recombinant human PCNA protein expressed by E.coli
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.4
Purification Method: Protein A/G Purified
Dilution: WB: 1:1000-2000
Calculated MW: 30kDa
Observed MW: 36kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only