PCNA Monoclonal Antibody
Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Catalogue Numbers: MB65969-50, MB65969-100
Sizes: 50µl, 100µl
Swiss-Prot: P12004
Host: Mouse
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Background: Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.
Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity: Recognizes endogenous levels of PCNA protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of PCNA expression in Hela (A), 293T (B), NIH3T3 (C), rat brain (D) whole cell lysates., Immunohistochemical analysis of PCNA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Bioworld Molecular Weight: ~ 30 kDa
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name: Proliferating cell nuclear antigen; PCNA; Cyclin
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human PCNA. The exact sequence is proprietary.
Conjugate: Unconjugated
Modification: Unmodified